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	Provedor de dados Rev. Bras. Ciênc. Avic. (2) Autor Yuan,X (2) Chang,Z (1) Deng,H (1) Li,C (1) Mehmood,K (1) Nabi,F (1) Rehman,MU (1) Wang,C (1) Wang,Q (1) Wu,X (1) Zhang,H (1) Zhou,D (1) Mais... Palavra-chave AQP2 (1) Avian Reovirus (1) Broiler chickens (1) Gene cloning (1) Interferon (1) Interferon-induced transmembrane protein 3 (1) Kidney (1) Prokaryotic expression (1) Renal dose dopamine (1) Yellow-feathered broiler (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2017 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Distribution of Ifitm3 in Yellow-Feathered Broilers and Inhibition of Avian Reovirus Multiplication by Ifitm3 Rev. Bras. Ciênc. Avic. Wang,Q; Yuan,X; Li,C; Deng,H; Wang,C. ABSTRACT This study was carried to express the interferon-induced transmembrane protein 3 (IFITM3) in vitro and examine its function in inhibition of avian reovirus (ARV) replication. The recombinant prokaryotic vector expressing yellow-feathered broiler IFITM3 was successfully constructed, and the recombinant protein was expressed in competent Escherichia coli BL21 cells. New Zealand white rabbits were immunized with the purified recombinant protein to prepare a polyclonal antibody, with a titer of 1:128,000. Immunohistochemistry, reverse transcription-PCR, and real-time fluorescence quantitative PCR showed that IFITM3 was distributed in the yellow-feathered broiler immune organs, and the expression of IFITM3 in bursa of Fabricius was more than in spleen... Tipo: Info:eu-repo/semantics/article Palavras-chave: Yellow-feathered broiler; Interferon; Interferon-induced transmembrane protein 3; Gene cloning; Prokaryotic expression; Avian Reovirus. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000200377 Renal Dose Dopamine Mediates the Level of Aquaporin-2 Water Channel (Aqp2) in Broiler Chickens Rev. Bras. Ciênc. Avic. Chang,Z; Zhang,H; Wu,X; Nabi,F; Rehman,MU; Yuan,X; Mehmood,K; Zhou,D. ABSTRACT Aquaporin 2 (AQP2) is a small protein located in the collecting tubules of kidneys; it plays an important role in the concentration and production of urine. The aim of this study was to determine the expression level of the AQP2 gene in the kidney of broiler chickens after the administration of renal­dose dopamine. Broiler chickens (25 days-old) were randomly divided into two groups (n=20 per group): intravenous administration of saline solution (control group) or renal-dose dopamine (dopamine group). The expression and localization of the AQP2 gene were evaluated by real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry (IHC), respectively. The protein level of AQP2 was analyzed by western blot analysis. The dopamine... Tipo: Info:eu-repo/semantics/article Palavras-chave: AQP2; Renal dose dopamine; Kidney; Broiler chickens. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300387 Registros recuperados: 2 Primeira ... 1 ... Última

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